Detection of Chlamydia trachomatis in urine samples by nucleic acid tests: comparison with culture and enzyme immunoassay of genital swab specimens
- 1 December 1997
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 35 (12) , 3355-7
- https://doi.org/10.1128/jcm.35.12.3355-3357.1997
Abstract
Two commercially available nucleic acid-based tests, ligase chain reaction (LCR; Abbott Laboratories) and PCR (Roche Diagnostics), for the detection of Chlamydia trachomatis in male and female urine samples were compared with culture and enzyme immunoassay (EIA) (Microtrak; Syva) for C. trachomatis detection in genital samples. The samples were collected from 1,005 patients who attended a sexually transmitted disease clinic. In this study population, the prevalence of the infection was 4%. Specimens which were reactive in any of the tests were retested with a different PCR test using primers directed against the major outer membrane protein gene. With a "gold standard" of a positive culture, or any other positive test result if it was confirmed by an independent test, the Roche PCR (95% sensitive, 99.9% specific) was more sensitive than the LCR (75% sensitive, 100% specific) (chi2, P < 0.0001) while both tests were more sensitive than culture (58% sensitive, 100% specific) or EIA (45% sensitive, 100% specific) (chi2, P < 0.001). The Roche PCR and Abbott LCR tests of urine identified 65% and 30% more positive patients, respectively, than did testing by culture of urethral or cervical specimens. Nucleic acid testing of urine specimens for C. trachomatis is a more sensitive and convenient method for the detection of genital infection.Keywords
This publication has 12 references indexed in Scilit:
- Comparison of DNA Amplification Methods for the Detection of Chlamydia trachomatis in First-Void Urine From Asymptomatic Military RecruitsSexually Transmitted Diseases, 1996
- Detection of Chlamydia trachomatis by ligase chain reaction compared with polymerase chain reaction and cell culture in urogenital specimens.Sexually Transmitted Infections, 1995
- Comparison of enzyme immunoassay antigen detection, nucleic acid hybridization and PCR assay in the diagnosis ofChlamydia trachomatis infectionEuropean Journal of Clinical Microbiology & Infectious Diseases, 1995
- Diagnosis of Chlamydia trachomatis genitourinary infection in women by ligase chain reaction assay of urineThe Lancet, 1995
- Chlamydia trachomatis and sexually transmitted diseaseBMJ, 1994
- Detection of Chlamydia trachomatis in First Catch Urine Samples From Symptomatic and Asymptomatic MalesSexually Transmitted Diseases, 1994
- Urine and the laboratory diagnosis of Chlamydia trachomatis in males.Sexually Transmitted Infections, 1993
- Specific amplification of a DNA sequence common to all Chlamydia trachomatis serovars using the polymerase chain reactionResearch in Microbiology, 1989
- A common plasmid of Chlamydia trachomatisPlasmid, 1986
- Primary Isolation of TRIC Organisms in HeLa 229 Cells Treated with DEAE-DextranThe Journal of Infectious Diseases, 1972