[125I]Iodonaphtylazide labeling selectively a cysteine residue in the F0 of the ATP‐synthase from E. coli is unsuitable for topographic studies of membrane proteins
- 22 August 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 160 (1-2) , 239-242
- https://doi.org/10.1016/0014-5793(83)80974-0
Abstract
The ATP synthase from E. coli was reacted with the hydrophobic photolabel [125I]iodonaphtylazide. Subunit b in the F0-part was selectively labelled. Label was traced back to the single cysteine21 in subunit b. Thus the reactive intermediate of INA generated by photolysis had a high preference for nucleophiles. Due to this high selectivity the detection of membrane spanning peptide segments by labelling with INA is not reliable.Keywords
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