Effects of synthetic media on the growth of Neurospora crassa isolates carrying genes for benomyl resistance and vinclozolin resistance

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Abstract
A vinclozolin‐resistant mutant (Vin‐1) was derived from a wild type strain of Neurospora crassa by ultraviolet mutagenesis, and the mutant was crossed with a benomyl‐resistant strain (Bml). Progeny carrying the four possiblecombinations of Bml, Bml+, Vin‐1 and Vin‐1+ genes were compared with respect to their radial growth on dishes of synthetic media. The benomyl‐resistant progeny Bml, Vin‐1+ were indistinguishable from the phenotypically normal progeny Bml+, Vin‐1+, and the vinclozolinresistant progeny Bml+, Vin‐1 were indistinguishable from the double mutants Bml, Vin‐1 on most media. Growth and sporulation of isolates carrying the mutant Vin‐1 gene were poor, especially on media of high osmotic pressure and on media enriched with ammonium salts or amino‐acids. Vinclozolin‐resistant isolates were also resistant to chloroneb, dicloran and procymidone. Possible effects of Vin genes on cell wall biogenesis and on the biological fitness of mutants are discussed.