Purification of αS1-Casein by Fast Protein Liquid Chromatography
Open Access
- 1 September 1989
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 72 (9) , 2242-2246
- https://doi.org/10.3168/jds.s0022-0302(89)79354-1
Abstract
An enriched .alpha.S1-casein was prepared by batch fractionation of whole casein on DEAE-cellulose using CaCl2 as eluent. The .alpha.s1-casein fraction was then submitted to hydrophobic interaction chromtography on a semipreparative fast protein liquid chromatography system. Electrophoretically pure .alpha.s1-casein was obtained by eluting with .05 M sodium phosphate at pH and containing 3.75 M urea.Keywords
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