The structural origin of the color differences in the bioluminescence of firefly luciferase
- 8 April 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 384 (1) , 83-86
- https://doi.org/10.1016/0014-5793(96)00288-8
Abstract
Six chimeric mutants between Hotaria parvula (λmax=568 nm) and Pyrocoelia miyako (λmax=550 nm) luciferases were reconstructed to determine the structural origin of the color differences in firefly luciferase. Based on light-emitting color, five chimeric luciferases could be divided into two groups: the three green-emitting mutants, classified as P. miyako luciferase, and the two yellow-emitting mutants, classified as H. parvula luciferase. Their common fragments between Val-209 and Ala-318 within each group contain the active site for the color differences.Keywords
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