Evidence that 3′‐phosphorylated polyphosphoinositides are generated at the nuclear surface: use of immunostaining technique with monoclonal antibodies specific for PI 3,4‐P2
Open Access
- 9 May 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 473 (2) , 222-226
- https://doi.org/10.1016/s0014-5793(00)01535-0
Abstract
Phosphatidylinositol (PI) 3,4‐P2 is a phosphoinositide that has been shown to be important for signal transduction in growth factor stimulation. We have produced monoclonal antibodies specific for PI 3,4‐P2, which were able to detect PI 3,4‐P2 generated in 293T cells treated with H2O2, or in MKN45/BD110 cells expressing activated PI 3‐kinase in immunostaining. Prolonged treatment with 0.05% Tween 20 resulted in detection of staining not only at the plasma membrane, but also at the nuclear surface, indicating that 3′‐phosphorylated phosphoinositides can be generated and function in the nucleus.Keywords
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