On-Column and Post-Column Derivatization for Capillary Electrophoresis with Laser-Induced Fluorescence for the Analysis of Single Cells
- 1 November 1995
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 18 (18-19) , 3557-3576
- https://doi.org/10.1080/10826079508014611
Abstract
Derivatization methods for analysis of single cell components by capillary electrophoresis with laser-induced fluorescence detection are reviewed. Recent advances have lead to the use of this technique for the identification of amino acids and proteins in many different cell lines ranging in size from 8 to 140 μm. Pre-column, on-column, and post-column methods of derivatization are examined in order to determine which results in the least amount of sample handling and dilution leading to better detection limits for the analytes of interest.Keywords
This publication has 47 references indexed in Scilit:
- Post-column derivatization in narrow-bore capillaries for the analysis of amino acids and proteins by capillary electrophoresis with fluorescence detectionJournal of Microcolumn Separations, 1994
- Quantitative determination of catecholamines in individual bovine adrenomedullary cells by reversed-phase microcolumn liquid chromatography with electrochemical detectionAnalytical Chemistry, 1992
- Capillary zone electrophoresis separation and laser-induced fluorescence detection of zeptomole quantities of fluorescein thiohydantoin derivatives of amino acidsTalanta, 1992
- High mass resolution secondary ion mass spectrometry via simultaneous detection with a charge-coupled deviceAnalytical Chemistry, 1991
- Quantitative amino acid analysis of individual snail neurons by open tubular liquid chromatographyAnalytical Chemistry, 1990
- Separation of serotonin from catechols by capillary zone electrophoresis with electrochemical detectionAnalytical Chemistry, 1989
- High speed capillary zone electrophoresis with laser induced fluorescence detectionJournal of High Resolution Chromatography, 1988
- Post-capillary fluorescence detection in capillary zone electrophoresis using o-phthaldialdehydeJournal of Chromatography A, 1988
- Chemical analysis of single neurons by open tubular liquid chromatographyMicrochimica Acta, 1987
- Neurochemical analysis of single neurons: A mini‐review dediated to oliver H. LowryJournal of Neuroscience Research, 1987