Human mitochondrial topoisomerase I
- 28 August 2001
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 98 (19) , 10608-10613
- https://doi.org/10.1073/pnas.191321998
Abstract
Tension generated in the circular mitochondrial genome during replication and transcription points to the need for mtDNA topoisomerase activity. Here we report a 601-aa polypeptide highly homologous to nuclear topoisomerase I. The N-terminal domain of this novel topoisomerase contains a mitochondrial localization sequence and lacks a nuclear localization signal. Therefore, we refer to this polypeptide as top1mt. The pattern of top1mt expression matches the requirement for high mitochondrial activity in specific tissues. top1mt is a type IB topoisomerase that requires divalent metal (Ca(2+) or Mg(2+)) and alkaline pH for optimum activity. The TOP1mt gene is highly homologous to the nuclear TOP1 gene and consists of 14 exons. It is localized on human chromosome 8q24.3.Keywords
This publication has 52 references indexed in Scilit:
- Mitochondrial disordersBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1999
- Localization by fluorescence in situ hybridization (FISH) of human mitochondrial polymerase γ (POLG) to human chromosome band 15q24→q26, and of mouse mitochondrial polymerase γ (Polg) to mouse chromosome band 7E, with confirmation by direct sequence analysis of bacterial artificial chromosomes (BACs)Cytogenetic and Genome Research, 1997
- On the Relationship of the ATP-Independent, Mitochondrial-Associated DNA Topoisomerase of Saccharomyces cerevisiae to the Nuclear Topoisomerase IBiochemical and Biophysical Research Communications, 1995
- Evidence for the Presence of Topoisomerase-like Activity in Mitochondria of Saccharomyces cerevisiaeBiochemical and Biophysical Research Communications, 1994
- Immunological idenTIFication of human platelet mitochondrial DNA topoisomerase IBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1993
- Mitochondrial DNA topoisomerase I from human plateletsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1991
- Purification and characterization of a type I DNA topoisomerase from calf thymus mitochondriaBiochemistry, 1987
- The presence of two mitochondrial DNA topoisomerases in human acute leukemia cellsBiochemical and Biophysical Research Communications, 1985
- Isolation of a mitochondrial DNA topoisomerase from human leukemia cellsBiochemical and Biophysical Research Communications, 1984
- Interaction between DNA and an Escherichia coli protein ωJournal of Molecular Biology, 1971