Polyol dehydrogenases. 3. Galactitol dehydrogenase and d-iditol dehydrogenase

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Abstract
A microorganism identified as a Pseudomonas sp. forms adaptive enzymes which oxidize a large number of polyols. The properties and specificities of the enzymes were studied in cell-free extracts. They are diphosphopyridine nucleotide-linked dehydrogenases converting polyols into ketoses. Sorbitol and dulcitol induce the formation of galactitol dehydrogenase, which oxidizes dulcitol to D-tagatose and accounts for the oxidation of other fully hydroxylated polyols containing an L-threo configuration adjacent to a primary alcohol group. Dulcitol also induces formation of a less stable enzyme (D-iditol dehydrogenase) which accounts for the oxidation of certain fully hydroxylated polyols containing a D-threo configuration adjacent to a primary alcohol group. A labile mannitol dehydrogenase occurs in the extracts of cells grown in media supplemented with sorbitol.