Transformation of Streptococcus bovis protoplasts by plasmid DNA
- 1 February 1996
- journal article
- Published by Wiley in Letters in Applied Microbiology
- Vol. 22 (2) , 159-161
- https://doi.org/10.1111/j.1472-765x.1996.tb01132.x
Abstract
The transformation and subsequent regeneration of ruminal strain Streptococcus bovis AO24/85 protoplasts by plasmid DNA was studied. The best stabilizer for regeneration of protoplasted cells was 5% sucrose in the regeneration medium and in the agar plates. Optimal concentration of polyethylene glycol 6000 in the transformation medium was 25% for both plasmids tested. Addition of Ca2+ and Mg2+ ions (2.5 mmol l-1) to the transformation medium increased the proportion of regenerated cells. Transformation frequencies were 3 x 10(3) transformants per microgram of pNZ12 and 2.4 x 10(2) per microgram of pJK108, respectively.Keywords
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