Isolation and RNA sequence analysis of cytochrome b mutants resistant to funiculosin, a center i inhibitor of the mitochondrial ubiquinol‐cytochrome c reductase in Saccharomyces cerevisiae

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Abstract
Funiculosin is a well‐known inhibitor of the mitochondrial respiratory chain, probably acting at the ubiquinone reducing site or center i of QH2 cytochrome c reductase. We report here the isolation, mapping and RNA sequence analysis of yeast apo‐cytochrome b mutants resistant to this inhibitor. Funiculosin‐resistance was found to be conferred, in 4 independent isolates, upon replacement of a leucine residue by phenylalanine in position 198 of the cytochrome b polypeptide chain.