Chloroplast DNA variation among diploid species in Brassica and allied genera.

Abstract
The restriction enzyme cleavage patterns of chloroplast DNA of eleven diploid species of Brassica and its allied genera [Brassica adpressa Boiss., Brassica campestris L., Brassica fruticulosa Cyril., Brassica nigra (L.) Koch, Brassica oleracea L., Brassica tournefortii Gouan, Diplotaxis erucoides (L.) DC., Eruca sativa Gars., Raphanus sativus L., Sinapis alba L., and Sinapis arvensis L.] were compared by using five restriction endonucleases. A dendrogram of species relationships as shown by the estimated numbers of recognition site mutations was drawn, the species being grouped into three clusters at the 5 recognition site mutations. The clusters did not always correspond to genera recognized in current taxonomy, while good correspondence was observed with the grouping by fraction I protein large subunits. The species relationships as seen with the present method were significantly correlated with those found in cytogenetical studies but not with those based on numerical morphological taxonomy.