In vitrostudies on long‐term stability of R plasmids inEscherichia coliK12
- 1 January 1989
- journal article
- research article
- Published by Wiley in Journal of Basic Microbiology
- Vol. 29 (10) , 643-653
- https://doi.org/10.1002/jobm.3620291002
Abstract
The maintenance and stability in anE. coliK12 host of environmental isolates of R plasmids encoding gentamicin resistance during multiple passages in antibiotic‐free and antibiotic‐containing broth were investigated in regard to the conferred resistance phenotypes and the respectiveEcoRI digestion patterns.Only two plasmids belonging to theIncM group maintained stable endonuclease digestion patterns over a 15‐month period in both of the media applied. Other members of this group revealed a considerable variability in theirEcoRI digestion patterns, but a stability in their resistance determinants. TwoIncM plasmids and anIncK plasmid exhibited partial resistance loss under nonselective conditions. The complete segregation of resistance determinants fromIncOF plasmids resulted in a stably maintained cryptic core plasmid.Keywords
This publication has 36 references indexed in Scilit:
- Persistence of an antibiotic resistance plasmid in intestinal Escherichia coli of chickens in the absence of selective pressureAntimicrobial Agents and Chemotherapy, 1987
- Spontaneous Deletions of Drug-Resistance Determinants from Salmonella Typhimurium in Escherichia ColiJournal of Medical Microbiology, 1986
- The molecular evolution of antibiotic resistance genes in Gram-negative bacteriaJournal of Antimicrobial Chemotherapy, 1986
- Molecular evolution, species distribution, and clinical consequences of an endemic aminoglycoside resistance plasmidAntimicrobial Agents and Chemotherapy, 1986
- Occurrence in Water and Waste Water of E. coli and Coliform Bacteria Carrying R‐Plasmids Part 3: Plasmids Encoding Gentamicin, Trimethoprim or Streptothricin ResistanceActa Hydrochimica et Hydrobiologica, 1986
- Transfer and stability of drug resistance plasmids inEscherichia coli K12Microbial Ecology, 1984
- Modular evolution of disseminated Inc 7-M plasmids encoding gentamicin resistancePlasmid, 1982
- A mutant of the plasmid R100.1 capable of producing autonomous circular forms of its resistance determinantPlasmid, 1982
- Molecular Analysis of R-Factors from Multiresistant Nosocomial IsolatesThe Journal of Infectious Diseases, 1980
- Survival of R-factor carryingEscherichia coliin mixed cultures in the chemostatFEMS Microbiology Letters, 1977