Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli.
- 1 November 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (11) , 4145-4149
- https://doi.org/10.1073/pnas.73.11.4145
Abstract
The thymidylate synthetase gene of B. subtilis bacteriophage .vphi.-3-T, when cloned in plasmids pSC101 or pMB9, is expressed in E. coli. The promoter of the cloned gene is likely to originate in .vphi.-3-T. Rearrangements of hybrid plasmid sequences during the cloning were noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of .vphi.-3-T, but not those of plasmid vectors.This publication has 14 references indexed in Scilit:
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