Mono-Substituted Vit. B12 Amides. II. Further Inhibition Study.

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Abstract
Summary The anilide, ethylamide, ethylamide monoacid, and di-(ethylamide) derivative of B12 inhibit cobalamin utilization by O. malhamensis and E. coli. The monobasic acid inhibits B12 utilization by E. coli, but not by O. malhamensis: the reverse is true for the dibasic acid. Methylamide and carbanilide derivatives are utilized as well as true B12; all compounds are as active as true B12 for E. gracilis and L. leichmannii. In E. coli, suboptimal levels of methionine in presence of B12 amides and acids give increasing growth with increasing concentrations of B12 derivatives; neither methionine nor B12 acid and amide, when added alone, permit growth.