Expression of VP5 of infectious pancreatic necrosis virus strain VR299 is initiated at the second in-frame start codon
- 1 April 2001
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 82 (4) , 805-812
- https://doi.org/10.1099/0022-1317-82-4-805
Abstract
Infectious pancreatic necrosis virus (IPNV), a member of the Birnaviridae with two double-stranded RNA genome segments, encodes five proteins designated VP1 to VP5. To study the function of the 17 kDa nonstructural protein VP5 during virus replication several mutated IPNV genome segments A were constructed and included in a reverse genetics system for IPNV to obtain recombinant virus. Mutations between nt 68 and 85 or nt 94 and 103 in the noncoding region failed to yield viable virus. Only mutations located between nt 86 and 92 and downstream of nt 104 were tolerated, and viable virus could be generated. All IPNV generated showed no difference in replication compared with the wild-type IPNV, indicating that the absence of expression of VP5 did not influence virus growth in vitro. Furthermore, the results presented here indicate that initiation of translation of VP5 occurs at position 113, the second in-frame start codon.Keywords
This publication has 34 references indexed in Scilit:
- Serological classification of infectious pancreatic necrosis (IPN) virus and other aquatic birnavirusesPublished by Elsevier ,2000
- A non-canonical Lon proteinase lacking the ATPase domain employs the Ser-Lys catalytic dyad to exercise broad control over the life cycle of a double-stranded RNA virusThe EMBO Journal, 2000
- Efficient Rescue of Infectious Bursal Disease Virus from Cloned cDNA: Evidence for Involvement of the 3′-Terminal Sequence in Genome ReplicationVirology, 1999
- Incomplete dsRNA Genomes in Purified Infectious Pancreatic Necrosis VirusVirology, 1997
- Genetic engineering of animal RNA virusesTrends in Microbiology, 1996
- Characterization of the small open reading frame on genome segment A of infectious pancreatic necrosis virusJournal of General Virology, 1995
- Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: A putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motifVirology, 1991
- Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.Proceedings of the National Academy of Sciences, 1988
- The nudeotide sequence of Infectious pancreatic necrosis virus (IPNV) dsRNA segment A reveals one large ORF encoding a precursor polyproteinNucleic Acids Research, 1986
- Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene.Proceedings of the National Academy of Sciences, 1982