Neutrophil labeling with indium-111: tropolone vs. oxine.

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Abstract
Tropolone was compared with oxine (8-hydroxy-quinoline) for labeling human neutrophils with 111IN. Exposure of neutrophils to tropolone at concentrations required for efficient labeling resulted in a marked impairment of chemotaxis. In contrast, no impairment of neutrophil chemotaxis was observed using 111In oxine. Labeling efficiencies obtained with 111In tropolone under optimal conditions were consistently less than those obtained with 111In oxine. Cells labeled by the 2 methods were evaluated using chemotaxis radioassay to assess the chemotatic potential of labeled cells. The oxine technique is preferable to tropolone for labeling human neutrophils with 111In.