The separation of amino acids and their N-acetyl derivatives by paper chromatography and paper ionophoresis

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Abstract
An apparatus for two-dimensional paper ionophoresis or for one-dimensional paper ionophoresis to be followed by paper chromatography in a 2d dimension is described. A method for determining the flow of electrolyte at different points on the paper is described. Equations expressing the relationship of the flow of electrolyte with experimental conditions of potential gradient, duration of current flow and the applied heating load are given. The effect of this movement of buffer on the paper on the separation of mixtures of ions or charged particles is discussed. A separation of 21 amino acids by two-dimensional ionophoresis is given. A separation of monoiodotyrosine, di-iodotyrosine, tri-iodothyrohine and thyroxine from 15 other amino acids and an inorganic iodide ion is described. A separation of 18 N-acetyl amino acids by one-dimensional paper ionophoresis followed by one-dimensional chromatography is also given.