The Modification of the Peptidyl Transferase Activity of 50-S Ribosomal Subunits, LiCl-Split Proteins and L16 Ribosomal Protein by Ethoxyformic Anhydride

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Abstract
Ethoxyformic anhydride abolishes the peptidyl transferase activity of 50‐S ribosomal subunits, LiCl split proteins and L16. Hydroxylamine treatment results in reactivation. Erythromycin exhibits significant protection with 50‐S ribosomal subunits. With LiCl split proteins and L16 significant protection was exhibited only after reconstitution. The results indicate that the ethoxyformic anhydride is reacting with approximately six histidines in LiCl split proteins and one in L16. Since L16 has been reported to contain a single histidine, the results presented indicate the involvement of this histidine in peptidyl transferase activity.