PHOSPHOETHANOLAMINE-INDUCED AND FRUCTOSE 1,6-DIPHOSPHATE-INDUCED CALCIUM-UPTAKE IN BONE FORMED INVITRO

  • 1 May 1987
    • journal article
    • research article
    • Vol. 2  (3) , 201-210
Abstract
The purpose of this investigation was to determine whether biologically available organic phosphates other than .beta.-glycerophosphate were capable of inducing mineralization of bone in vitro. The chick periosteal osteogenesis model was used to demonstrate that endogenously available organic phosphates, fructose 1,6-diphosphate (F1,6-D) and phosphoethanolamine (PEA) induced calcium accumulation in bone formed in vitro. Alkaline phosphatase activity was inhibited in a dose-dependent manner by PEA, and the sodium salt of F1,6-D. There was an inverse correlation between alkaline phosphatase activity and organic phosphate-mediated mineralization. The data demonstrate that certain biologically available organic phosphates can induce mineralization and modulate bone metabolism in vitro.