Rate of RNA synthesis and tRNA end-labeling during early development of Phaseolus

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Abstract
Axes of Phaseolus vulgaris cease synthesis of RNA during the maturation stage of embryogeny. During the imbibition phase of germination RNA synthesis resumes after the axes reach a normal water content. In the first hour of inbibition a very low rate of incorporation of 3H-adenosine into is RNA found, and the primary site of incorporation is the-CCA end of tRNA. At later stages of germination tRNA end-labeling accounts for a minor fraction of adenosine incorporation. The rate of RNA synthesis increases after initiation of axis elongation to a maximal rate at 18 h of germination. ATP pool-size and specific activity vary over a several-fold range during development, an important consideration in determining the rate of RNA synthesis.