NUCLEAR-PORES AND DNA PLOIDY IN HUMAN BLADDER CARCINOMAS

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (9) , 3752-3756
Abstract
The number of nuclear pores per .mu.m2 was determined on the freeze-fractured nuclei of 20 human bladder tumors and 5 control samples of normal bladder epithelium. Measurements of the nuclear surface and volume were also performed and the mean number of pores per nucleus and the ratio of pore to volume were calculated. The DNA distribution pattern on the same samples was determined by flow cytometry. All control samples and 12 tumors were diploid and 8 tumors were aneuploid. The mean number of pores per .mu.m2 and mean total number of pores per nucleus in the control samples and in diploid tumors were similar. In the aneuploid tumors, both values were significantly higher. However, the ratio of pore to volume was shown to be constant regardless of the DNA content. It was further observed that, in aneuploid tumors, there are 2 populations of nuclei, 1 with density of pores similar to the diploid tumor and 1 with a higher pore density. Because aneuploid bladder tumors were shown to have more aggressive behavior than diploid tumors, increased density of nuclear pores or their total number per nucleus may be related to tumor behavior. This view is supported by the observation that 5 of 8 tumors with increased density and total pore number were invasive, while all tumors with low pore number were noninvasive.