FACTORS AFFECTING THE GROWTH OF PROPIONIBACTERIA

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Abstract
Growth of 4 strains of propionibacteria is delayed or suppressed altogether when small inocula are introduced into media to which glucose is added aseptically after autoclaving of the other medium constituents. Prompt growth results when the carbohydrate is autoclaved with the medium. Other reducing sugars such as fructose and mannose are as effective as glucose. This growth stimulating effect can be duplicated by autoclaving sugar free media at a more acid pH (5) for short periods of time (10 minutes) or at the usual pH (6.7) for longer periods (30 minutes). Under certain conditions, N-D-glucosylglycine partially replaces the growth enhancement produced by autoclaving glucose with the medium.