Cultural and Physiological Observations on Trypanosoma rhodesiense and Trypanosoma gambiense

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Abstract
A diphasic medium allowing indefinite cultivation is descr. The solid base contains rabbit blood; human blood does not in general show any advantage, with the possible exception of primary isolation. The cultures are noninfective, because the parasites develop only to the proventri-cular stage. The parasites of both spp. are aerobic fermenters; they consume sugar and produce acid. The living flagellates produce very little, if any, ammonia.
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