Demonstration of cyclic AMP in bone cells by immuno-histochemical methods
- 1 December 1975
- journal article
- research article
- Published by Springer Nature in Calcified Tissue International
- Vol. 19 (1) , 305-315
- https://doi.org/10.1007/bf02564013
Abstract
By utilizing immunohistochemical procedures it was possible to locate bone cells that contained adenosine 3′5′-monophosphate (cyclic AMP) in frozen, undecalcified cat jaw sections. An immunoglobulin-enzyme bridge method was found to be superior to other techniques, including immunofluorescence. Differences in staining pattern were found between bone cells of kittens and young adult cats, indicating different metabolic levels related to cellular maturity, and different intracellular compartmentalization of cyclic AMP in the two age groups.This publication has 14 references indexed in Scilit:
- The measurement of adenosine 3′,5′-monophosphate in boneAnalytical Biochemistry, 1975
- Cyclic Guanosine and Adenosine 3′,5′-Monophosphates in Canine Thyroid: Localization by ImmunofluorescenceScience, 1974
- Adenosine 3′,5′-Monophosphate Is Localized in Cerebellar Neurons: Immunofluorescence EvidenceScience, 1972
- A METHOD FOR DETECTING INTRACELLULAR CYCLIC ADENOSINE MONOPHOSPHATE BY IMMUNOFLUORESCENCEJournal of Histochemistry & Cytochemistry, 1972
- A Method for Combined Microradiography and Histological Analysis of Non-Decalcified Hard TissuesActa Odontologica Scandinavica, 1972
- RADIOIMMUNOASSAY FOR THE MEASUREMENT OF ADENOSINE 3′,5′-CYCLIC PHOSPHATEProceedings of the National Academy of Sciences, 1969
- AN IMMUNOGLOBULIN-ENZYME BRIDGE METHOD FOR LOCALIZING TISSUE ANTIGENSJournal of Histochemistry & Cytochemistry, 1969
- Selective enzyme purification by affinity chromatography.Proceedings of the National Academy of Sciences, 1968
- ENZYME-LABELED ANTIBODIES: PREPARATION AND APPLICATION FOR THE LOCALIZATION OF ANTIGENSJournal of Histochemistry & Cytochemistry, 1966
- THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUEJournal of Histochemistry & Cytochemistry, 1966