Deacetyl-thymosin .BETA.4: Synthesis and effect on the impaired peripheral T-cell subsets in patients with chronic renal failure.

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Abstract
Deacetyl-thymosin .beta.4 was synthesized by successive azide condensations of 5 peptide fragments, Boc-(1-4)-NHNH2, Boc-(5-12)-NHNH2, Boc-(13-20)-NHNH2, Boc-(21-27)-NHNH2 and Boc-(28-33)-NHNH2, with H-(34-43)-OBzl, followed by deprotection with HF in the presence of anisole and thianisole. The deprotected peptide was incubated with dithiothreitol to reduce sulfoxide on the Meth side chain. The synthetic deacetyl-thymosin .beta.4 increased almost the entire peripheral T-cell population and a helper T-cell subset when incubated in vitro with uremic patient''s blood but a suppressor T-cell subset was unaffected under these conditions.