Localization of factor VIIIC: antigen in guinea‐pig tissues and isolated liver cell fractions

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Abstract
Summary. Factor VIIIC: antigen (VIII: CAg) was estimated in guinea-pig tissues by an immunoradiometric assay using a human inhibitor antibody. In homogenized guinea-pig tissues, VIII: CAg was shown to be stable and to be predominantly located in the liver (9±1·2 units; mean ±SEM, n= 8). Lesser amounts were detected in spleen (1·3±0·02 units), lung (0·6±0·07) and kidney (0·4±0·06). In isolated liver cell fractions separated by centrifugal elutriation VIII: CAg was mainly detected in the hepatocyte fraction (0·3±0·07 units/108 cells; mean ± SEM, n= 5) and in lesser amounts in the endothelial (0·02±0·01 units/108 cells) and the Kupffer cell fractions (0·05±0·02 units/108 cells). The liver concentration of VIII:CAg was (0·17±0·02 units/g) which was 20% of the plasma concentration (0·96±0·01 units/ml, n= 8) suggesting that VIII: CAg may not be stored in the liver but is rapidly exported following synthesis.