Improvements in the method of determining individual phospholipids in a complex mixture by successive chemical hydrolyses

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Abstract
Modifications are described of the method (Dawson, 1960) of determining individual phos-pholipids in a complex mixture by successive chemical hydrolyses. These extend its coverage to include phosphatidyl glycerol, phosphatidyl-inositomannosides, the higher phosphoinositides and the alkyl ether phospholipids. The recovery of individual plasmalogens is greatly improved by hydrolysing them to glycerylphosphoryl-base derivatives with a trichloroacetic acid-mercuric chloride reagent, which reduces the formation of cyclic acetals to a minimum. Complete analyses are presented for the phospholipid distribution in human erythrocyte stroma and ox heart, liver and brain.