Direct selection of human genomic loci by microarray hybridization
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- 14 October 2007
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 4 (11) , 903-905
- https://doi.org/10.1038/nmeth1111
Abstract
We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.Keywords
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