Theory of single-molecule fluorescence spectroscopy of two-state systems
- 8 May 1999
- journal article
- research article
- Published by AIP Publishing in The Journal of Chemical Physics
- Vol. 110 (18) , 9145-9150
- https://doi.org/10.1063/1.478836
Abstract
We derive an explicit expression for the probability density of the amplitudes of the biexponential fluorescence decay of a two-state system observed in single-molecule experiments of finite duration T. The shape of the probability density of the amplitudes depends on (k 1 +k 2 )T, where the k i are the interconversion rate constants. It approaches a delta function in the limit T→∞. When the interconversion rates in the ground and excited states differ, the single-molecule and bulk experiments will give different results even in the limit of infinite duration of the experiment.Keywords
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