Temperature Effects on Lymphocyte TransformationIn Vitro

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Abstract
Phytohemagglutinin (PHA)-induced transformation of normal rat peripheral lymphocytes has been studied at a wide range of culture temperatures (4d`C to 42d`C). Lymphocyte transformation was maximum at 37d`C while insignificant stimulation was observed between 4d`C and 30d`C. Temperatures above 37d`C produced sub-optimal transformation as measured by synthesis of DNA and protein, and appearance of lymphoblasts. Binding studies using 125I-PHA indicate that the low temperature inhibition of lymphocyte transformation could be a result of excess lectin (being available as a result of low temperature) bound to the cell surface, preventing the initiation of the molecular events associated with transformation.