ALDOSTERONE STIMULATION IN VITRO

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Abstract
The effect of rat serum and serum fractions on biosynthesis of aldosterone, corticosterone and deoxycorticosterone was investigated by a previously described in vitro assay procedure, using adrenal sections from rats which had been kept on a sodium-deficient diet. Addition of small amounts of serum to the incubation medium significantly stimulated aldosterone and deoxycorticosterone production. An almost linear log-dose/response curve was obtained over a 1:100 concentration range. Stimulation of corticosterone biosynthesis was observed only at high concentrations of serum. Whereas most of the aldosterone- and deoxycorticosterone-stimulating activities were dialysable, most of the corticosterone-stimulating activity remained in the non-dialysable fraction. Ion-exchange and gel filtration chromatography indicated that the unknown dialysable aldosterone-stimulating substance was different from the known aldosterone-stimulating agents, i. e. angiotensin II and monovalent cations.