Genotypic variations among isolates ofMarasmius quercophilus, a white-rot fungus isolated from evergreen oak litter

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Abstract
Marasmius quercophilus is a white-rot basidiomycete isolated from evergreen oak litter. This fungus plays an extensive role in litter degradation because of its cellulase, pectinase, and laccase production. Our objective was to investigate the potential genotypic polymorphism of this species using RAPD (random amplified polymorphic DNA) and the amplification and the sequencing of fragments ITS (internal transcribed spacer) from the rRNA repeat. An intraspecies polymorphism was observed for the M. quercophilus isolates studied as suggested by the tree found with RAPD data: seven subgroups were defined. The minimum similarity coefficient observed in these groups was 0.278. The sizes of fragments ITS 1 and ITS 2 were the same for each isolate (ITS 1: 320 bp and ITS 2: 450 bp), and the percentages of nucleotide variation were 1.25 and 1.11%, respectively. Few isolates from the studied population had the same RAPD pattern that would suggest a clonal development in some zones of the site of La Gardiole.Key words: Marasmius quercophilus, intraspecific variability, ITS, RAPD.
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