Isolation of Model Soluble Immune Complexes in the Fluid Phase by Monoclonal Anti‐IgG‐ Ferritin Antibodies
- 1 January 1985
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 21 (1) , 1-9
- https://doi.org/10.1111/j.1365-3083.1985.tb01396.x
Abstract
Ferritin conjugates of monoclonal IgG anti-human .gamma. chain (anti-IgG-F) were reacted with soluble heat aggregates of IgG (A-IgG) and with soluble DNA-anti-DNA complexes to increase the S rate of the model soluble immune complexes (ICx) and thus facilitate isolation of ICx in the fluid phase and provide an immunochemical marker for subsequent ultrastructural analysis. A-IgG appeared as globular or curvilinear structures with individual IgG molecules arranged in a random fashion. The technique appears promising for characterization of other soluble ICx.This publication has 23 references indexed in Scilit:
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