Vitamin E Supplementation of High Fat Diets for Dairy Cows

Open Access
Publisher Website
Google Scholar
Abstract
High fat diets .+-. 1 g .alpha.-tocopheryl acetate per head per day were fed to 28 cows for 4 mo. to determine whether dietary fat increases dietary requirement for vitamin E. Tocopherol supplementation did not affect milk production or composition. After 4 mo. there was no difference between treatment groups for oxidative stability (thiobarbituric acid test) of milk stored for 5 days with .1 ppm added Cu, but milk of supplemented cows was more stable when 1 ppm Cu was added. Oxidative stability was normal in the unsupplemented group. Tocopherol concentration of blood plasma increased and Se concentration decreased with tocopherol supplementation; activity of glutathione peroxidase was lower in plasma, whole blood, and erythrocytes of the supplemented group. Tissue integrity was unchanged by tocopherol supplementation, as estimated by erythrocyte hemolytic resistance and activities of lactic dehydrogenase and glutamic oxalacetic transaminase in blood serum. Clearance of bromosulphthalein from blood plasma was less for cows fed fat and supplemented with tocopherol than for control cows fed neither fat nor vitamin E. No indication of impaired vitamin E status was observed when 4% stabilized fat was added to the diet.