A NEW CONTINUOUS CELL LINE FROM LARVAE OF THE MOSQUITO AEDES ALBOPICTUS (DIPTERA, CULICIDAE)

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Abstract
A continuous cell line was established from the 1st instar larvae of a mosquito, A. albopictus. Primary cultures were prepared from pieces of larval bodies. Cells of various shapes migrated from the explanted larval fragments and multiplied on the glass surface of the culture vessel. The 1st subculture was made 53 days after the culture was set up and 100 passages were made during the following 625 days. The established cell line was designated as NIAS-AeA1-2. The mode of chromosome number of the cell line was 6 (2n). Population doubling time was .apprx. 30 h at 25.degree. C. Cells were stored at 5.degree. C for .apprx. 2 mo. The cells consumed aspartic acid and glutamic acid more actively than other mosquito cell lines. Cultures were apparently free from contamination by mycoplasmas and viruses. The cells were susceptible to Chilo iridescnet virus (Iridoviridae). The cell line could be cultured continuously in serum-free culture media.