FERRITIN SYNTHESIS IN PERIPHERAL-BLOOD MONOCYTES IN IDIOPATHIC HEMOCHROMATOSIS

Google Scholar
  • 1 January 1982
    • journal article
    • research article
    • Vol. 100  (1) , 137-145
Abstract
RE [reticuloendothelial] ferritin synthesis in IHC [idiopathic hemoichromatosis] was studied in .**GRAPHIC**. [monocytes] isolated from peripheral blood. Phlebotomized hemochromatosis patients (12) with normal body Fe stores and healthy control subjects (12) were studied. .**GRAPHIC**. in culture were exposed to Fe as either ferric ammonium citrate or Fe dextran, and ferritin synthesis was determined by measurement of 3H-leucine incorporation after isolation of ferritin by a quantitative immunoadsorbent technique. Fe at concentrations of 150 and 300 .mu.M induced de novo ferritin synthesis, increasing both 3H-leucine incorporation into ferritin and cellular ferritin content. No differences were observed between hemochromatosis and control .**GRAPHIC**. with respect to basal or Fe-stimulated ferritin synthesis. .**GRAPHIC**. ferritin synthesis in IHC does not differ from .**GRAPHIC**. ferritin synthesis in normal subjects, and the reported abnormalities in RE Fe storage in IHC are therefore not related to an inability of RE cells to synthesize ferritin.