Purification and Characterization of Hepatic Microsomal Cytochrome P‐450 in Phenobarbital‐ and β‐Naphthoflavone‐Treated Pigs

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Abstract
Different cytochrome P‐450 isoenzymes from hepatic microsomes of phenobarbital (PB) and β‐naphthoflavone (β‐NF) treated pigs and rats were isolated, purified, and characterized. The physico‐chemical properties of the porcine isoenzymes were similar to properties of forms isolated from other species. The molecular sizes ranged from 52.5 to 59.5 kD and, in the ferrous‐carbonyl state, the isoenzymes had absorbance maxima between 447 and 451 nm. Antigenic similarities were found between the isoenzymes present in PB‐induced pigs, and between the isoenzymes present in β‐NF‐induced pigs. Cross‐reactivity was not observed between PB‐ and β‐NF‐inducible isoenzymes, but β‐NF‐inducible isoenzymes in pigs and rats possessed antigenic similarities.