Alpha-l-arabinofuranosidase from Rhodotorula flava

Abstract
An .alpha.-L-arabinosuranosidase (EC 3.2.1.55) from the culture fluid of R. flava IFO 0407 grown on beet arabinan as a C source was highly purified. The purified enzyme has a pH optimum of 2.0. The enzyme is unusually acid stable retaining 82% of its activity after being maintained for 24 h at pH 1.5 and at 30.degree. C. The apparent Km and Vmax values of the enzyme for phenyl .alpha.-L-arabinofuranoside were determined to be 9.1 mM and 72.5 .mu.mol/min per mg of protein, respectively.

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