Direct Molecular Analysis of Whole-Body Animal Tissue Sections by MALDI Imaging Mass Spectrometry
- 5 July 2010
- book chapter
- Published by Springer Nature
- Vol. 656, 285-301
- https://doi.org/10.1007/978-1-60761-746-4_17
Abstract
The determination of the localization of various compounds in a whole animal is valuable for many applications, including pharmaceutical absorption, distribution, metabolism, and excretion (ADME) studies and biomarker discovery. Imaging mass spectrometry is a powerful tool for localizing compounds of biological interest with molecular specificity and relatively high resolution. Utilizing imaging mass spectrometry for whole-body animal sections offers considerable analytical advantages compared to traditional methods, such as whole-body autoradiography, but the experiment is not straightforward. This chapter addresses the advantages and unique challenges that the application of imaging mass spectrometry to whole-body animal sections entails, including discussions of sample preparation, matrix application, signal normalization, and image generation. Lipid and protein images obtained from whole-body tissue sections of mouse pups are presented along with detailed protocols for the experiments.Keywords
This publication has 19 references indexed in Scilit:
- Enhancement of protein sensitivity for MALDI imaging mass spectrometry after chemical treatment of tissue sectionsJournal of the American Society for Mass Spectrometry, 2008
- Solvent-Free Matrix Dry-Coating for MALDI Imaging of PhospholipidsJournal of the American Society for Mass Spectrometry, 2008
- Molecular MALDI imaging: An emerging technology for neuroscience studiesDevelopmental Neurobiology, 2008
- Sublimation as a method of matrix application for mass spectrometric imagingJournal of the American Society for Mass Spectrometry, 2007
- MALDI-MS-based imaging of small molecules and proteins in tissuesCurrent Opinion in Chemical Biology, 2007
- Processing MALDI mass spectra to improve mass spectral direct tissue analysisInternational Journal of Mass Spectrometry, 2006
- Investigation of the profiling depth in matrix‐assisted laser desorption/ionization imaging mass spectrometryRapid Communications in Mass Spectrometry, 2005
- Integrating Histology and Imaging Mass SpectrometryAnalytical Chemistry, 2004
- Direct tissue analysis using matrix‐assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparationJournal of Mass Spectrometry, 2003
- Cryosectioning distortion reduction using tape supportMicroscopy Research and Technique, 2001