Polypeptide composition of higher plant photosystem I complex
- 24 April 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 263 (2) , 274-278
- https://doi.org/10.1016/0014-5793(90)81391-z
Abstract
High resolution gel electrophoresis of the native photosystem I complex retaining light-harvesting chlorophyll complex revealed the presence of three low-molecular-mass proteins of 7, 4.1 and 3.9 kDa in spinach, and 6.8, 4.4 and 4.1 kDa in pea, in addition to the other well-characterized higher-molecular-mass components. Upon further detergent treatment to deplete light-harvesting chlorophyll complex, the 7 kDa and 4.1 kDa proteins were removed from the photosystem I core complex of spinach, while the 3.9 kDa protein was retained. N-terminal sequencing demonstrated that the 4.1 kDa proteins from both spinach and pea correspond to the gene product of ORF42/44 in chloroplast genome of liverwort and higher plants, which was previously hypothesized as a photosystem I gene ( psa J) based on sequence homology with the cyanobacterial photosystem I component of 4.1 kDa [(1989) FEBS Lett. 253, 257-263]. N-terminal sequence of the spinach 3.9 kDa and pea 4.4 kDa proteins fitted with chloroplast ORF36/40 ( psa I) although no homologue has been found in cyanobacteria. The spinach 7 kDa and pea 6.8 kDa proteins correspond to the nuclear-encoded psa K product and significantly matched with the N-terminal sequence of the cyanobacterial 6.5 kDa subunit. The evolutional conservation of the psa J and psa K seems to suggest their intrinsic role(s) in photosystem I.Keywords
This publication has 33 references indexed in Scilit:
- Characterization and N‐terminal sequence of a 5 kDa polypeptide in the photosystem I core complex from spinachFEBS Letters, 1989
- Identification of photosystem I components from the cyanobacterium, Synechococcus vulcanus by N‐terminal sequencingFEBS Letters, 1989
- Identification of psbI and psbL gene products in cyanobacterial photosystem II reaction center preparationFEBS Letters, 1989
- A cDNA clone encoding the precursor for a 10.2 kDa photosystem I polypeptide of barleyFEBS Letters, 1989
- N‐terminal sequencing of photosystem II low‐molecular‐mass proteins 5 and 4.1 kDa components of the O2‐evolving core complex from higher plantsFEBS Letters, 1989
- A cDNA clone encoding a 10.8 kDa photosystem I polypeptide of barleyFEBS Letters, 1988
- Nucleotide sequence of cDNA clones encoding the entire precursor polypeptides for subunits IV and V of the photosystem I reaction center from spinachFEBS Letters, 1988
- Cloning and sequencing of spinach cDNA clones encoding the 20 kDa PS I polypeptideFEBS Letters, 1988
- The 8 kDa polypeptide in photosystem I is a probable candidate of an iron‐sulfur center protein coded by the chloroplast gene frxAFEBS Letters, 1987
- Structure, function and organization of the photosystem I reaction center complexBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1987