Nucleotide sequence and expression of aPseudomonas savastanoicytokinin biosynthetic gene: homotogy withAgrobacterium tumefaciens tmrandtzsloci
- 11 March 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (6) , 2555-2565
- https://doi.org/10.1093/nar/14.6.2555
Abstract
The nucleotide sequence of a Pseudomonas trans-zetatin producing gene (ptz) from the pCKl plasmid of Pseudomonas syringae pv. savastanoi strain 1006 has been determined. This gene confers upon E. coli the ability to synthesize and secrete several cytokinins including trans-zeatin, iso-pentenyladenine and their respective N 9 -ribosyl derivatives. Sequence analysis indicates an open reading frame encoding a protein of 234 amino acids with a molecular weight of 26,816. Significant sequence homology is found between ptz and both the tzs and tmr genes from Agrobacterium tumefaciens. The results suggest a close relationship between the cytokinin biosynthetic pathways in P. savastanoi and A tumefaciens.Keywords
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