Reactivity of modified ribose moleties of guanosine: new cleavage reactions mediated by the IVS ofTetrahymenaprecursor rRNA
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (4) , 1559-1577
- https://doi.org/10.1093/nar/15.4.1559
Abstract
An RNA molecule consisting of the 5' exon and intervening sequence (IVS) of Tetrahymena precursor rRNA was oxidized with sodium periodate to convert the ribose moiety of the 3' terminal guanosine into a dialdehyde form. The modified RNA undergoes a specific cleavage reaction at the 5' splice site, but has no apparent cyclization activity. This novel reaction mediated by the IVS RNA is pH dependent over the range 6.5-8.5 and leaves a 5' phosphate and a 3'-OH at the newly created termini. The dialdehyde form of monomer guanosine is also capable of causing a specific cleavage reaction at the 5' splice site although the nucleotide is not covalently attached to the IVS RNA in the final product. These and other findings described in this report suggest that the cis diol of the intact ribose moiety of guanosine is not an absolute requirement for the IVS-mediated reactions.Keywords
This publication has 18 references indexed in Scilit:
- One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activityCell, 1986
- The Tetrahymena intervening sequence ribonucleic acid enzyme is a phosphotransferase and an acid phosphataseBiochemistry, 1986
- New reactions of the ribosomal RNA precursor of Tetrahymena and the mechanism of self-splicingJournal of Molecular Biology, 1986
- Reactions of the intervening sequence of the Tetrahymena ribosomal ribonucleic acid precursor: pH dependence of cyclization and site-specific hydrolysisBiochemistry, 1985
- Reversibility of cyclization of the tetrahymena rRNA intervening sequence: implication for the mechanism of splice site choiceCell, 1985
- Ribonuclease P Catalysis Differs from Ribosomal RNA Self-SplicingScience, 1985
- Template-Directed Synthesis of Novel, Nucleic Acid-Like StructuresScience, 1985
- Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage–ligation reactionNature, 1983
- Making ends meet: a model for RNA splicing in fungal mitochondriaNature, 1982
- Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structureBiochimie, 1982