Rapid Screening for the Isolation of Mutants of Aspergillus nidulans with Increased Penicillin Yields
Open Access
- 1 December 1974
- journal article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 37 (4) , 515-523
- https://doi.org/10.1111/j.1365-2672.1974.tb00476.x
Abstract
Summary. After UV treatment conidia of a strain of Aspergillus nidulans were plated on an agar medium. The survivors gave rise to individual colonies which were inoculated separately on agar discs and incubated. The discs were then transferred to biological assay plates seeded with spores of a strain of Bacillus subtilis sensitive to penicillin. Using this primary screening method, mutants have been isolated which, when tested later in shake flask cultures, gave larger penicillin yields than the parent strains.Keywords
This publication has 3 references indexed in Scilit:
- Improvement of kasugamycin-producing strain by the agar piece method and the prototroph methodFolia Microbiologica, 1971
- A REVISED MAP OF THE EIGHT LINKAGE GROUPS OF ASPERGILLUS NIDULANSGenetics, 1967
- Isolation of Auxotrophs of Penicillium chrysogenum and their Penicillin YieldsJournal of General Microbiology, 1963