Effects of Parathyroid Hormone and Calcitonin on Alkaline Phosphatase Activity and Matrix Calcification in Rabbit Growth-Plate Chondrocyte Cultures
- 1 July 1990
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 127 (1) , 114-118
- https://doi.org/10.1210/endo-127-1-114
Abstract
The effects of PTH and calcitonin (CT) on the expression of mineralization-related phenotypes by chrondrocytes were examined. In cultures of pelleted growth-plate chondrocytes. PTH caused 60–90% decreases in alkaline phosphatase activity, the incorporation of 45Ca into insoluble material, and the calcium content during the post-mitotic stage. These effects of PTH were dose-dependent and reversible. In contrast, CT increased alkaline phosphatase activity, 45Ca incorporation into insoluble material, and the calcium content by 1.4- to 1.8-fold. These observations suggest that PTH directly inhibits the expression of the mineralization-related phenotypes by growthplate chondrocytes, and that CT has the opposite effects. (Endocrinology127: 114–118,1990)Keywords
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