Dietary coenzyme Q10 supplement renders swine hearts resistant to ischemia-reperfusion injury
- 1 April 2000
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Heart and Circulatory Physiology
- Vol. 278 (4) , H1084-H1090
- https://doi.org/10.1152/ajpheart.2000.278.4.h1084
Abstract
To examine whether nutritional supplementation of coenzyme Q10(CoQ10) can reduce myocardial ischemia-reperfusion injury, a group of swine was fed a regular diet supplemented with CoQ10 (5 mg ⋅ kg− 1 ⋅ day− 1) for 30 days. Another group of pigs that were fed a regular diet supplemented with placebo served as a control. After 30 days, isolated in situ pig hearts were prepared and hearts were perfused with a cardiopulmonary pump system. Each heart was subjected to 15 min of regional ischemia by snaring of the left anterior descending coronary artery, followed by 60 min of hypothermic cardioplegic global ischemia and 120 min of reperfusion. After the experiments were completed, myocardial infarct size was measured by triphenyltrazolium chloride staining methods. Postischemic left ventricular contractile function was better recovered in the CoQ10 group than in the control group of pigs. CoQ10-fed pigs revealed less myocardial infarction and less creatine kinase release from the coronary effluent compared with control pigs. The experimental group also demonstrated a smaller amount of malonaldehyde in the coronary effluent and a higher content of the endogenous antioxidants ascorbate and thiol. Significant induction of the expression of ubiquitin mRNA was also found in the hearts of the CoQ10-fed group. The results of this study demonstrate that nutritional supplementation of CoQ10 renders the hearts resistant to ischemia-reperfusion injury, probably by reducing the oxidative stress.Keywords
This publication has 24 references indexed in Scilit:
- Ubiquitin-conjugating enzymes: novel regulators of eukaryotic cellsPublished by Elsevier ,2003
- Biochemical, physiological and medical aspects of ubiquinone functionPublished by Elsevier ,1999
- Detection of Oxidative DNA Damage to Ischemic Reperfused Rat Hearts by 8-Hydroxydeoxyguanosine FormationJournal of Molecular and Cellular Cardiology, 1998
- Induction of the Haem Oxygenase Gene Expression during the Reperfusion of Ischemic Rat MyocardiumJournal of Molecular and Cellular Cardiology, 1996
- Detection of oxidative stress in heart by estimating the dinitrophenylhydrazine derivative of malonaldehydeJournal of Molecular and Cellular Cardiology, 1995
- Lipid Peroxidation‐Derived Free Radical Production and Postischemic Myocardial Reperfusion InjuryaAnnals of the New York Academy of Sciences, 1994
- THE UBIQUITIN SYSTEM FOR PROTEIN DEGRADATIONAnnual Review of Biochemistry, 1992
- Ratio of Low-Density Lipoprotein Cholesterol to Ubiquinone as a Coronary Risk FactorNew England Journal of Medicine, 1991
- Pathophysiology of superoxide radical as potential mediator of reperfusion injury in pig heartBasic Research in Cardiology, 1986
- Improvement in recovery of left ventricular function during reperfusion with coenzyme Q10 in isolated working rat heartCardiovascular Research, 1985