Intervening sequences in a Dictyostelium gene that encodes a low abundance class mRNA
Open Access
- 1 January 1980
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (23) , 5599-5610
- https://doi.org/10.1093/nar/8.23.5599
Abstract
Using S1 nuclease protection experiments and DNA sequencing, we have identified two intervening sequences (introns) within a Dictyostelium gene that codes for a low abundance class mRNA. The two introns are located within the protein coding region of this gene. Both are small ( ∼100bp) and extremely (∼95%) A + T rich. The splice junction sequences are similar to the splice sites in other eukaryotes. Finally, we have shown that these introns are transcribed as part of a higher molecular weight nuclear precursor.Keywords
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