The biosynthesis of raffinose

Abstract
Reaction of UTP and [alpha]- D-galactose 1-phosphate with [U-C14] sucrose in the presence of a Vicia faba dormant-seed preparation yielded the trisaccharide raffinose. UTP-[alpha]-D-galactose 1-phosphate-uridylyltransferase activity has been demonstrated in the bean preparation and evidence for the participation of UDP-galactose in the trisaccharide synthesis is presented. UDP-galacttose 4-epimerase is present in the dormant seed. The biosynthesis of raffinose in relation to the metabolism of other carbohydrates in plants is discussed.

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