Direct evidence for chromosomal inversion during T-cell receptor β-gene rearrangements
- 1 January 1986
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 319 (6048) , 28-33
- https://doi.org/10.1038/319028a0
Abstract
A germline T-cell receptor variable region (Vβ) gene segment (Vβ14) has been mapped 10 kilobases to the 3′ side of the constant region (Cβ2) gene. The Vβ14 gene segment is in an inverted transcriptional polarity relative to the diversity-region (Dβ) and joining-region (Jβ) gene segments and the Cβ genes. Analyses of a T-cell clone (J6.19), which has productively rearranged the Vβ14 gene segment, indicate that the productive Vβ-Dβ-Jβ rearrangement and its reciprocal flank recombination product are linked and located at either border of a chromosomal inversion. These data demonstrate for the first time a linkage between mammalian V and C genes and verify that a functional T-cell receptor Vβ gene can be constructed through a chromosomal inversion.Keywords
This publication has 40 references indexed in Scilit:
- Location of Gene for β Subunit of Human T-Cell Receptor at Band 7q35, a Region Prone to Rearrangements in T CellsScience, 1985
- Rearranged β t cell receptor genes in a helper t cell clone specific for lysozyme: No correlation between Vβ and MHC restrictionCell, 1985
- Rearrangement and transcription of the β-chain genes of the T-cell antigen receptor in different types of murine lymphocytesNature, 1985
- Structure, expression and divergence of T-cell receptor β-chain variable regionsNature, 1984
- The structure, rearrangement and expression of Dβ gene segments of the murine T-cell antigen receptorNature, 1984
- Genomic organization and sequence of T-cell receptor β-chain constant- and joining-region genesNature, 1984
- Lambda replacement vectors carrying polylinker sequencesJournal of Molecular Biology, 1983
- Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptorNature, 1983
- Continuing kappa-gene rearrangement in a cell line transformed by Abelson murine leukemia virusCell, 1982
- Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genesNature, 1981