An Automated Method for Determination of Total Bile Acid Concentration in Canine Serum

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Abstract
The purpose of the study was to evaluate an automated modification of the enzymatic method, Enzabile (Nycomed) for the determination of total bile acid concentration in canine serum. Evaluation of the automated method was accomplished by measuring the within-run and between-day variation, by assessment of accuracy, and by assessment of detectability of the method. The automated method was found to correlate well with the original Enzabile (Nycomed) method. The resulting regression equation was found to be: y = 5.81 + 1.09 x, r = 0.99. Also, the variations and the detection limit of the automated method were smaller, respectively lower, compared to the reference method.